Siyuan Song1,2,3 · Yumin Yuan1,2,3 · Lingling Xu1,2,3 · Jun Jiang1,2,3 · Ying Li1,2,3 · Yao Yan1,2,3 · Qing Li1,2,3,4 · Fang Zhou1,2,3,4 · Junli Cao1,2,3 · Licai Zhang1,2,31 Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou 221004, China
2 Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou 221004, China
3 NMPA Key Laboratory for Research and Evaluation of Narcotic and Psychotropic Drugs, Xuzhou 221008, China
4 School of Nursing, Xuzhou Medical University, Xuzhou 221004, China
Abstract
We previously identified a unique nucleus, the cerebrospinal fluid (CSF)-contacting nucleus. This study aims to understand its gene architecture and preliminarily suggest its functions. The results showed that there were about 19,666 genes in this nucleus, of which 913 were distinct from the dorsal raphe nucleus (non-CSF contacting). The top 40 highly-expressed genes are mainly related to energy metabolism, protein synthesis, transport, secretion, and hydrolysis. The main neurotransmitter is 5-HT. The receptors of 5-HT and GABA are abundant. The channels for Cl–, Na+, K+, and Ca2+ are routinely expressed. The signaling molecules associated with the CaMK, JAK, and MAPK pathways were identified accurately. In particular, the channels of transient receptor potential associated with nociceptors and the solute carrier superfamily members associated with cell membrane transport were significantly expressed. The relationship between the main genes of the nucleus and life activities is preliminarily verified.
Keywords
Gene architecture; Cerebrospinal fuidcontacting nucleus (CSF-contacting nucleus); Biological function
