Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by 1 H-NMR Dynamic Mapping Method

Taotao Liu 1,2 • Zhengqian Li1 • Jindan He1 • Ning Yang1 • Dengyang Han1 • Yue Li1 • Xuebi Tian3 • Huili Liu2 • Anne Manyande4 • Hongbing Xiang3 • Fuqiang Xu 2,5 • Jie Wang 2,5,6 • Xiangyang Guo 1


Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China

2 Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China

3 Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

4 School of Human and Social Sciences, University of West London, Middlesex TW89GA, UK

5 University of Chinese Academy of Sciences, Beijing 100049, China

6 The Second Hospital of Shijiazhuang, Shijiazhuang 050051, China

Abstract

Abnormal postoperative neurobehavioral performance (APNP) is a common phenomenon in the early postoperative period. The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP. The dynamic changes of metabolites in different brain regions after anesthesia and surgery, as well as their potential association with APNP are still not well understood. Here, we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice, and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance (1H-NMR) spectroscopy. The abnormal neurobehavioral performance occurred at 6 h and/or 9 h, and recovered at 24 h after anesthesia/surgery. Compared with the control group, the altered metabolite of the model group at 6 h was aspartate (Asp), and the difference was mainly displayed in the cortex; while significant changes at 9 h occurred predominantly in the cortex and hippocampus, and the corresponding metabolites were Asp and glutamate (Glu). All changes returned to baseline at 24 h. The altered metabolic changes could have occurred as a result of the acute APNP, and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process.

Keywords

Abnormal postoperative neurobehavioral performance; 1H-NMR; Metabolite; Aspartate; Glutamate

[SpringerLink]

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